An Updated Overview of Protein Tags
A guide to recent and growing trends in protein tags
Introduction
Protein tagging has been around for decades, but their traditional uses are not frequent enough to make them common lab knowledge. Recent improvements in biochemical techniques have been increasing their frequency as creative design and analysis tools. In this overview, we offer a guide for the variety of different protein tags that are trending in recent growth.
This guide will cover:
- Principle Affinity Tags
- Biotin Tags
- Epitope Tags
- Fluorescent Tags
Principle Affinity Tags
Arg-tag
His-tag
GST
Biotin Tags
Strep-tag
Streptavadin-tag
Epitope Tags
c-myc-tag
FLAG-tag
HA-tag
Fluorescent Tags
Luciferase
GFP
S-tag
Points to Consider
- FLAG mAb purification systems have not always been stable and often require expert intervention.
- The presence of C-terminal tags can potentially contribute to a protein's loss of function. Particularly in the case of enzymes.
- Streptavadin-binding peptide (SBP) is only attachable to the C-terminus
- S-tag in low pH elution may alter protein properties and limit the matrix reproducibility
- anti-myc antibody antibody purification is inconsistent and has been proven to result in poor yields
References
- Nilsson et al. (1997a)
- Lazzaroni et al. (1985)
- Goldstein et al. (1992)
- Crespo et al. (1997)
- Makrides (1996)
- McLean et al. (2001)
- Nilsson et al. (1997b)
- Wang et al. (1996)
- Podbielski et al. (1992)
- Jones et al. (1995)
- Morandi et al. (1984)
- Kaldalu et al. (2000)
- Jones et al. (1995)
- Rubinfeld et al. (1991)
- Smith (2000)
- Gerdes and Kaether (1996)
- Los et al. (2008)
- Terpe (2003)
- Imagawa et al. (1982)
- Fritze and Anderson (2000)
- Tai et al. (1988)
- Kuliopulos and Walsh (1994)
- Kwatra et al. (1995)
- Tai et al. (1988)
- Karp and Oker-Blom (1999)
- Kolodziej and Young (1991)
- Terpe (2003)
- Kimple and Sondek (2002)
- Stevens (2000)
- Bornhorst and Falke (2000)
- Stevens (2000)
- Abdulaev et al. (2005), Biao et al. (2004), Ruan et al. (2004)
- Fritze and Anderson (2000)
- Berlot (1999)
- Nilsson et al. (1997b)
- Skerra and Schmidt (2000)
- Sano et al. (1998)
- Terpe (2003)
- Stevens (2000)
- Li (2011)
- Terpe (2003)
- Nelson et al. (1999)